Delineation of a 1Mb breakpoint region at 1p13 in Wilms tumors by fine‐tiling oligonucleotide array CGH
Identifieur interne : 002499 ( Main/Exploration ); précédent : 002498; suivant : 002500Delineation of a 1Mb breakpoint region at 1p13 in Wilms tumors by fine‐tiling oligonucleotide array CGH
Auteurs : Rachael Natrajan [Royaume-Uni] ; Richard D. Williams [Royaume-Uni] ; Anita Grigoriadis [Royaume-Uni] ; Alan Mackay [Royaume-Uni] ; Kerry Fenwick [Royaume-Uni] ; Alan Ashworth [Royaume-Uni] ; Jeffrey S. Dome [États-Unis] ; Paul E. Grundy [Canada] ; Kathy Pritchard-Jones [Royaume-Uni] ; Chris Jones [Royaume-Uni]Source :
- [ 1045-2257 ]
Abstract
Wilms tumor karyotypes frequently exhibit recurrent, large‐scale chromosomal imbalances, among the most common of which are concurrent loss of 1p and gain of 1q. We have previously identified a novel breakpoint at 1p13 by 1 Mb‐spaced array CGH, and have now undertaken a fine‐tiling oligonucleotide array approach to map the region accurately in four tumors exhibiting rearrangements at this locus. The use of a 10 bp‐spaced platform revealed that all four tumors in fact harbored different breakpoints, which targeted intragenic sequences in PHTF1, DCLRE1B, and NRAS, and an intergenic region immediately downstream of TRIM33. All four genes and breakpoints were within the 1.78 Mb intervals identified by the genome‐wide BAC arrays. The precise breakpoint interval was in each case mapped to a 200–1,200 bp region and was confirmed for one case to lie within intron 3 of DCLRE1B by quantitative PCR. Analysis of local genome architecture revealed no convincing conservation of repetitive sequences or specific translocation/recombination‐associated elements within the breakpoint regions. This study highlights the power of fine‐tiling oligonucleotide arrays to delineate breakpoint regions identified by genome‐wide screens. © 2007 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/gcc.20446
Affiliations:
- Canada, Royaume-Uni, États-Unis
- Angleterre, District de Columbia, Grand Londres
- Londres, Washington (district de Columbia)
- University College de Londres
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We have previously identified a novel breakpoint at 1p13 by 1 Mb‐spaced array CGH, and have now undertaken a fine‐tiling oligonucleotide array approach to map the region accurately in four tumors exhibiting rearrangements at this locus. The use of a 10 bp‐spaced platform revealed that all four tumors in fact harbored different breakpoints, which targeted intragenic sequences in PHTF1, DCLRE1B, and NRAS, and an intergenic region immediately downstream of TRIM33. All four genes and breakpoints were within the 1.78 Mb intervals identified by the genome‐wide BAC arrays. The precise breakpoint interval was in each case mapped to a 200–1,200 bp region and was confirmed for one case to lie within intron 3 of DCLRE1B by quantitative PCR. Analysis of local genome architecture revealed no convincing conservation of repetitive sequences or specific translocation/recombination‐associated elements within the breakpoint regions. This study highlights the power of fine‐tiling oligonucleotide arrays to delineate breakpoint regions identified by genome‐wide screens. © 2007 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Canada</li><li>Royaume-Uni</li><li>États-Unis</li></country><region><li>Angleterre</li><li>District de Columbia</li><li>Grand Londres</li></region><settlement><li>Londres</li><li>Washington (district de Columbia)</li></settlement><orgName><li>University College de Londres</li></orgName></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Natrajan, Rachael" sort="Natrajan, Rachael" uniqKey="Natrajan R" first="Rachael" last="Natrajan">Rachael Natrajan</name></noRegion><name sortKey="Ashworth, Alan" sort="Ashworth, Alan" uniqKey="Ashworth A" first="Alan" last="Ashworth">Alan Ashworth</name><name sortKey="Fenwick, Kerry" sort="Fenwick, Kerry" uniqKey="Fenwick K" first="Kerry" last="Fenwick">Kerry Fenwick</name><name sortKey="Grigoriadis, Anita" sort="Grigoriadis, Anita" uniqKey="Grigoriadis A" first="Anita" last="Grigoriadis">Anita Grigoriadis</name><name sortKey="Jones, Chris" sort="Jones, Chris" uniqKey="Jones C" first="Chris" last="Jones">Chris Jones</name><name sortKey="Jones, Chris" sort="Jones, Chris" uniqKey="Jones C" first="Chris" last="Jones">Chris Jones</name><name sortKey="Mackay, Alan" sort="Mackay, Alan" uniqKey="Mackay A" first="Alan" last="Mackay">Alan Mackay</name><name sortKey="Pritchard Ones, Kathy" sort="Pritchard Ones, Kathy" uniqKey="Pritchard Ones K" first="Kathy" last="Pritchard-Jones">Kathy Pritchard-Jones</name><name sortKey="Williams, Richard D" sort="Williams, Richard D" uniqKey="Williams R" first="Richard D." last="Williams">Richard D. Williams</name></country><country name="États-Unis"><region name="District de Columbia"><name sortKey="Dome, Jeffrey S" sort="Dome, Jeffrey S" uniqKey="Dome J" first="Jeffrey S." last="Dome">Jeffrey S. Dome</name></region></country><country name="Canada"><noRegion><name sortKey="Grundy, Paul E" sort="Grundy, Paul E" uniqKey="Grundy P" first="Paul E." last="Grundy">Paul E. Grundy</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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